Repository of Research and Investigative Information

Repository of Research and Investigative Information

Dezful University of Medical Sciences

Performance of Wayne assay for detection of pyrazinamide resistance in Mycobacterium tuberculosis: a meta-analysis study

(2021) Performance of Wayne assay for detection of pyrazinamide resistance in Mycobacterium tuberculosis: a meta-analysis study. New Microbes and New Infections. p. 8.

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Official URL: <Go to ISI>://WOS:000679975200012


Conventional culture-based drug susceptibility testing (DST) of Mycobacterium tuberculosis to pyrazinamide (PZA) is time-consuming and difficult to perform. The current systematic review and meta-analysis was aimed to evaluate the diagnostic accuracy of Wayne assay against culture-based DSTs as the reference standard. We searched the MEDLINE/Pubmed, Embase, and Web of Science databases for the relevant records. The QUADAS-2 tool was used to assess the quality of the studies. Diagnostic accuracy measures (i.e., sensitivity and specificity) were pooled with a random-effects model. Statistical analyses were performed with STATA (version 14, Stata Corporation, College Station, TX, USA), RevMan (version 5.3; The Nordic Cochrane Centre, the Cochrane Collaboration, Copenhagen, Denmark), and Meta-DiSc (version 1.4, Cochrane Colloquium, Barcelona, Spain). A total of 31 articles comprising data for 2457 isolates of M. tuberculosis were included in the final analysis. The pooled sensitivity and specificity of the Wayne assay against all reference tests (the combination of BACTEC MGIT 960, BACTEC 460, and proportion method) were 86.6 (95 CI: 84.3-88.7) and 96.0 (95 CI: 94.8-97). The positive likelihood ratio (PLR), negative likelihood ratio (NLR), diagnostic odds ratio (DOR), and the area under the curve (AUC) estimates were found to be 17.6 (95 CI: 10.5-29.3), 0.11 (95 CI: 0.06-0.20), 164 (95 CI: 83-320) and 97, respectively. Deek's test result indicated no evidence for publication bias (P > 0.05). Although the current study shows that the Wayne test is sensitive and specific for detecting PZA resistance, it may be used in combination with conventional DSTs to diagnose PZA resistance accurately. (c) 2021 The Author(s). Published by Elsevier Ltd.

Item Type: Article
Keywords: Pyrazinamide resistance systematic review tuberculosis Wayne assay drug susceptibility assay pnca mutations rapid detection mgit 960 gene system region Infectious Diseases Microbiology
Page Range: p. 8
Journal or Publication Title: New Microbes and New Infections
Journal Index: ISI
Volume: 42
Identification Number:
Depositing User: مهندس مهدی شریفی

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