Repository of Research and Investigative Information

Repository of Research and Investigative Information

Dezful University of Medical Sciences

Bioinformatics features and immunogenic epitopes of Echinococcus granulosus Myophilin as a promising target for vaccination against cystic echinococcosis

(2021) Bioinformatics features and immunogenic epitopes of Echinococcus granulosus Myophilin as a promising target for vaccination against cystic echinococcosis. Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases. p. 104714. ISSN 1567-7257 (Electronic) 1567-1348 (Linking)

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Official URL: https://www.ncbi.nlm.nih.gov/pubmed/33434702

Abstract

Cystic echinococcosis (CE) is a neglected zoonosis especially in underdeveloped countries around the world. Hence, immunization strategies are beneficial to avert the infection. The present investigation was aimed to predict the primary biochemical characteristics of the EgMyophilin and its potential B-cell and human leukocyte antigen (HLA)-binding epitopes as a promising vaccine candidate. Different web servers were used to predict physico-chemical, antigenic and allergenic profiles, transmembrane domain, subcellular localization, post-translational modification (PTM) sites, secondary and 3D structure, tertiary model refinement and validations. B-cell and HLA-binding epitopes were predicted and screened in terms antigenicity, allergenicity, solubility (B-cell) or hydrophobicity (T-cell). The 89.82 KDa protein was non-allergenic, hydrophilic, stable, with improved thermotolerance and 94 post-translational modification sites. The secondary structure included 42.94 alpha helix, 42.82 random coil and 41.23 extended strand. Based on Ramachandran plot output for refined model, 96.2, 99.5, and 0.45 of amino acid residues were incorporated in the favored, allowed, and outlier regions of the refined model, respectively. After epitope screening, four B-cell and five HLA-binding epitopes possessed the highest antigenic index in the protein sequence. This paper is a premise for further researches, and provides insights for the development of a suitable vaccine against CE. More empirical studies are required using the EgMyophilin alone or in combination with other antigens/epitopes in the future.

Item Type: Article
Keywords: Echinococcus granulosus Immunoinformatics Myophilin Vaccination
Subjects: QW Microbiology and Immunology > QW 501-949 Immunology
WC Communicable Diseases > WC 195-425 Infection. Bacterial Infections
Divisions: Education Vice-Chancellor Department > Faculty of Health > Department of Epidemiology
Page Range: p. 104714
Journal or Publication Title: Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases
Journal Index: Pubmed
Volume: 89
Identification Number: https://doi.org/10.1016/j.meegid.2021.104714
ISSN: 1567-7257 (Electronic) 1567-1348 (Linking)
Depositing User: مهندس مهدی شریفی
URI: http://eprints.dums.ac.ir/id/eprint/763

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