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Dezful University of Medical Sciences

Celecoxib inhibits acute edema and inflammatory biomarkers through peroxisome proliferator-activated receptor-γ in rats

(2020) Celecoxib inhibits acute edema and inflammatory biomarkers through peroxisome proliferator-activated receptor-γ in rats. Iranian Journal of Basic Medical Sciences. pp. 1544-1550. ISSN 20083866 (ISSN)

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Abstract

Objective(s): Celecoxib (CLX), a selective cyclooxygenase-II (COX-2) inhibitor, has been used for management of several inflammatory disorders. The present study aimed to explore the role of peroxisome proliferator-activated receptor-gamma (PPARγ) in CLX induced anti-inflammatory response in rats. Materials and Methods: Carrageenan-induced paw edema was used as an acute inflammation model. Rats were treated with various intra-peritoneal (IP) doses of CLX (0.3-30 mg/kg) and pioglitazone (PGL; PPARγ agonist, 1-20 mg/kg) alone or in combination. Amounts of PPARγ, COX-2, and prostaglandin E2 (PGE2) in paw tissue, and extents of TNF-α and IL-10 in serum were measured. Moreover, levels of oxidative stress parameters as malondialdehyde (MDA), glutathione (GSH), glutathione peroxidase (GPx) activity in the cortex, hippocampus, and paw tissues were also determined. Results: CLX and PGL dose-dependent administration (IP), alone or in combination reduced carrageenan-induced paw edema. Further, both agents, alone or in combination, reduced either the amounts of COX-2, PGE2, and MDA in the inflamed paw, and the levels of TNF-α in serum which were elevated by carrageenan. Both drugs also increased both levels of PPARγ, GSH, GPx activity in paws, and serum levels of IL-10 that were decreased by carrageenan. Intraplantar injection of GW-9662 (IPL), a selective PPARγ antagonist, inhibited all biochemical modifications caused by both single and combined drug treatments. Conclusion: CLX produced its anti-inflammatory effects probably through PPARγ receptor activation. Besides, increased anti-inflammatory effects of CLX with PGL suggest that their combination might be applied for the clinical management of inflammation especially in patients suffering from diabetes. © 2020 Mashhad University of Medical Sciences. All rights reserved.

Item Type: Article
Keywords: Carrageenan Celecoxib Cytokines Oxidative stress Pioglitazone PPAR-γ Rat
Subjects: QS Human Anatomy > QS504-532 Histology
QU Biochemistry. Cell Biology and Genetics > QU 300-560 Cell Biology and Genetics
QV Pharmacology > QV 120-140 Autonomic Agents. Nonmetallic Elements. Neuromuscular Agents
QY Clinical Laboratory Pathology > QY 50-60 Laboratory Animals
Divisions: Education Vice-Chancellor Department > Faculty of Pharmacy > Department of Pharmacognosy and Pharmaceutical Biotechnology
Page Range: pp. 1544-1550
Journal or Publication Title: Iranian Journal of Basic Medical Sciences
Journal Index: Scopus
Volume: 23
Number: 12
Identification Number: https://doi.org/10.22038/ijbms.2020.43995.10315
ISSN: 20083866 (ISSN)
Depositing User: مهندس مهدی شریفی
URI: http://eprints.dums.ac.ir/id/eprint/728

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