Repository of Research and Investigative Information

Repository of Research and Investigative Information

Dezful University of Medical Sciences

Changes in nitric oxide synthase levels are associated with impaired cardiac function and tolerance to ischemia-reperfusion injury in male rats with transient congenital hypothyroidism

(2020) Changes in nitric oxide synthase levels are associated with impaired cardiac function and tolerance to ischemia-reperfusion injury in male rats with transient congenital hypothyroidism. Naunyn-Schmiedebergs Archives of Pharmacology. p. 9. ISSN 0028-1298

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Abstract

Transient congenital hypothyroidism (TCH) has long-lasting consequences on the cardiovascular system during adulthood. The aim of this study was to determine whether nitric oxide (NO) and NO-producing enzymes are involved in impaired cardiac function as well as decreased tolerance to ischemia-reperfusion (IR) injury in adult male rats with TCH. Pregnant rats were divided into control and hypothyroid groups. Male offspring rats were categorized in control and hypothyroid (TCH) groups at week 16. Levels of NOx (nitrate+nitrite) and neuronal NOS (nNOS), inducible NOS (iNOS), and endothelial NOS (eNOS) were measured in hearts of rats and isolated perfused hearts from both groups were subjected to IR. Levels of NOx and NOSs were also measured in both groups after ischemia. Compared with controls, heart NOx levels were higher at baseline (48.0 +/- 4.9 vs. 35.0 +/- 2.6 mu mol/L; P = 0.034) and following IR (103.6 +/- 4.2 vs. 70.2 +/- 2.7 mu mol/L; P < 0.001) in rat with TCH. At baseline, compared with controls, heart iNOS and nNOS levels were significantly higher in rats with TCH (6.12 +/- 0.34 vs. 4.78 +/- 0.27 ng/mg protein; P = 0.008 for iNOS and 4.87 +/- 0.28 vs. 3.55 +/- 0.23 ng/mg protein; P = 0.003 for nNOS). Following IR, in rats with TCH, heart iNOS levels increased (11.75 +/- 2.02 vs. 6.12 +/- 0.34, ng/mg protein; P = 0.015) whereas nNOS level decreased (4.10 +/- 0.25 vs. 4.87 +/- 0.28 ng/mg protein; P = 0.063). Adverse effects of TCH on cardiac function are associated with increased ratio of iNOS/eNOS; in addition, increased heart nNOS levels are involved in impaired cardiac function while its decrease is associated with decreased tolerance to IR injury.

Item Type: Article
Keywords: Transient congenital hypothyroidism Nitric oxide Cardiac function Neuronal NOS Inducible NOS Endothelial NOS fetal hypothyroidism myocardial-ischemia gender-differences coronary-arteries gene-expression adult rats heart no overexpression cardioprotection Pharmacology & Pharmacy
Subjects: QU Biochemistry. Cell Biology and Genetics > QU 100-133 Biochemistry of the Human Body
Divisions: Education Vice-Chancellor Department > Faculty of Medicine > Department of Basic Science > Department of Clinical Biochemistry
Page Range: p. 9
Journal or Publication Title: Naunyn-Schmiedebergs Archives of Pharmacology
Journal Index: ISI
Identification Number: https://doi.org/10.1007/s00210-020-01812-6
ISSN: 0028-1298
Depositing User: مهندس مهدی شریفی
URI: http://eprints.dums.ac.ir/id/eprint/569

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