Repository of Research and Investigative Information

Repository of Research and Investigative Information

Dezful University of Medical Sciences

Expression and clinicopathological significance of lipin-1 in human breast cancer and its association with p53 tumor suppressor gene

(2020) Expression and clinicopathological significance of lipin-1 in human breast cancer and its association with p53 tumor suppressor gene. Journal of Cellular Physiology. p. 12. ISSN 0021-9541

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Abstract

Breast cancer (BC) is an important cause of female cancer-related death. It has recently been demonstrated that metabolic disorders including lipid metabolism are a hallmark of cancer cells. Lipin-1 is an enzyme that displays phosphatidate phosphatase activity and regulates the rate-limiting step in the pathway of triglycerides and phospholipids synthesis. The objective of this study was to evaluate lipin-1 expression, its prognostic significance, and its correlation with p53 tumor suppressor in patients with BC. In this study, 55 pairs of fresh samples of BC and adjacent noncancerous tissue were used to analyze lipin-1, using quantitative real-time polymerase chain reaction and immunohistochemistry (IHC) staining. The expression of other clinicopathological variables and p53 was also examined using IHC technique. The cell migration was studied in MCF-7 and MDA-MB231 cells following the inhibition of lipin-1 by propranolol. Our results show that the relative expression of lipin-1 messenger RNA was significantly higher in BC tissues compared with the adjacent normal tissue and its inhibition reduced cell migration in cancer cells. This upregulation was negatively correlated with histological grade of tumor and p53 status (p = .001 and p = .034) respectively and positively correlated with the tumor size (p = .006). Our results also seem to indicate that the high lipin-1 expression is related to a good prognosis in patients with BC. The expression of lipin-1 may be considered as a novel independent prognostic factor. The inhibition of lipin-1 may also have therapeutic significance for patients with BC. The correlation between lipin-1 and p53 confirms the role of p53 in the regulation of lipid metabolism in cancer cells.

Item Type: Article
Keywords: breast cancer lipin-1 p53 tumor suppressor metabolism inhibition prognosis survival protein storage marker Cell Biology Physiology
Subjects: QW Microbiology and Immunology > QW 501-949 Immunology
Divisions: Education Vice-Chancellor Department > Faculty of Medicine > Department of Basic Science > Immunology Department
Page Range: p. 12
Journal or Publication Title: Journal of Cellular Physiology
Journal Index: ISI
Identification Number: https://doi.org/10.1002/jcp.29523
ISSN: 0021-9541
Depositing User: مهندس مهدی شریفی
URI: http://eprints.dums.ac.ir/id/eprint/567

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