Repository of Research and Investigative Information

Repository of Research and Investigative Information

Dezful University of Medical Sciences

In vitro modulation of natural killer activity of human peripheral blood mononuclear cells against prostate tumor cell line

(2011) In vitro modulation of natural killer activity of human peripheral blood mononuclear cells against prostate tumor cell line. Immunopharmacology and Immunotoxicology. pp. 700-708. ISSN 08923973 (ISSN)

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Objective: Natural killer (NK) cells have long been known to be involved in the recognition and lysis of tumor cells but the mechanisms contributing to deficient NK activity in patients with cancer remains unclear. Manipulation of them is likely essential to the success of cancer immunotherapy protocols although optimal stimulation and maintenance of NK activity remains elusive. Here we studied the stimulatory effects of PHA and K562, on NK activity of human peripheral blood mononuclear cells (PBMCs). Materials and methods: The NK activity of PBMCs against DU-145 was determined with 51Cr-release assay. The PBMCs were stimulated with PHA, either on one occasion or repetitively on three occasions (1-PHA-PBMC or 3-PHA-PBMC, respectively), and were incubated with irradiated K562 (iK562). The expression of CD56, NKG2D and MICA/B were detected on PBMCs and cell lines with flow cytometry. Results: PHA stimulation increased the proportion of CD56+ cells and upregulated NKG2D expression on 1-PHA-PBMC and 3-PHA-PBMC, but co-incubation with iK562 decreased NKG2D expression on 1-PHA-PBMC without change of NKG2D expression on the 3-PHA-PBMC. NK activity of 1-PHA-PBMC appeared to decrease with co-incubation with iK562 compared to a significant increase in activity of 3-PHA-PBMC. A similar increase in interferon-γ (IFN-γ) secretion from 3-PHA-PBMC was demonstrated compared to 1-PHA-PBMC. Discussion and conclusion: Our results demonstrated that varying the mitogen exposure to PBMCs affect the influence of iK562 on NK activity. This effect appeared to be unrelated to the subsequent expression of NKG2D or IFN-γ secretion. These results may be beneficial in the development of future cancer immunotherapy protocols. © 2011 Informa Healthcare USA, Inc.

Item Type: Article
Keywords: IFN-γ Immunomodulation Natural killer activity NKG2D PHA CD3 antigen CD56 antigen gamma interferon k 562 mica b protein natural killer cell receptor NKG2D phytohemagglutinin protein unclassified drug article cell activity cell stimulation cell strain K 562 chromium 51 release controlled study cytokine release down regulation flow cytometry human human cell irradiation male natural killer cell priority journal prostate tumor protein expression tumor cell upregulation Antigens, Differentiation Humans Immunity, Cellular Interferon-gamma K562 Cells Killer Cells, Natural Leukocytes, Mononuclear Mitogens Phytohemagglutinins Prostatic Neoplasms
Page Range: pp. 700-708
Journal or Publication Title: Immunopharmacology and Immunotoxicology
Journal Index: Scopus
Volume: 33
Number: 4
Identification Number:
ISSN: 08923973 (ISSN)
Depositing User: مهندس مهدی شریفی

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