Repository of Research and Investigative Information

Repository of Research and Investigative Information

Dezful University of Medical Sciences

Lactobacilus Delbrueckii subsp. Bulgaricus Modulates the Secretion of Th1/Th2 and Treg Cell-Related Cytokines by PBMCs from Patients with Atopic Dermatitis

(2017) Lactobacilus Delbrueckii subsp. Bulgaricus Modulates the Secretion of Th1/Th2 and Treg Cell-Related Cytokines by PBMCs from Patients with Atopic Dermatitis. Drug Research. pp. 724-729. ISSN 2194-9379

Full text not available from this repository.

Official URL: http://apps.webofknowledge.com/InboundService.do?F...

Abstract

Background Atopic dermatitis (AD) is an inflammatory skin disease which may be due to the imbalance between Th1-, Th2 and Treg cell-related immune responses. Evidences suggest that appropriate stimulation with probiotics may correct the skewed immune response in children with AD. The aim was to determine the effects of the yogurt culture lactobacillus Bulgaricus on the secretion of Th1/Th2/Treg type cytokines by PBMCs from children with AD. Methods L. Bulgaricus was cultivated on MRS broth. The PBMCs from 20 children with AD were separated by Ficoll-Hypaque centrifugation and co-cultured with different concentrations of UV killed bacteria in RPMI-1640 plus 10 FCS for 48/72 h. The levels of IL-10, IL-4, IL-12 and IFN-gamma were measured in supernatant of PBMCs by ELISA. Results L. Bulgaricus significantly up-regulated the secretion of IL-10, IL-12 and IFN-gamma, whereas decreased the secretion of IL-4 by PBMCs at both incubation times 48 h/72 h and both bacteria: PBMCs ratios 100:1/50:1, compared to control (p < 0.05). There were no significant differences between incubation times 48 h and 72 h regarding the secretion levels of IL-12, IFN-gamma and IL-4. However, the secretion of IL-10 by L. Bulgaricus-stimulated PBMCs at incubation time 72 h and in the presence of bacteria: PBMCs ratio 100:1 was significantly higher than in incubation time 48 h and in the presence of bacteria: PBMCs ratio 50:1 (P < 0.000 and P < 0.00, respectively). Conclusion These data show that L. Bulgaricus may modulate the secretion of Th1-, Th2-Treg-related cytokines in AD patients. Therefore, the possible potential therapeutic of L. Bulgaricus for treatment of AD should be consider in further investigation.

Item Type: Article
Keywords: atopic dermatitis probiotics lactobacillus bulgaricus Th1 Th2 treg cytokines blood mononuclear-cells lactic-acid bacteria probiotics expression children disease eczema infection cheese Pharmacology & Pharmacy Chemistry
Page Range: pp. 724-729
Journal or Publication Title: Drug Research
Journal Index: ISI
Volume: 67
Number: 12
Identification Number: https://doi.org/10.1055/s-0043-117612
ISSN: 2194-9379
Depositing User: مهندس مهدی شریفی
URI: http://eprints.dums.ac.ir/id/eprint/227

Actions (login required)

View Item View Item