Repository of Research and Investigative Information

Repository of Research and Investigative Information

Dezful University of Medical Sciences

Investigating the cytotoxic effect of chamomile aqueous extract on 4T1 and 47D cells and level of caspase3 protein in breast cancer cells T-47D

(2018) Investigating the cytotoxic effect of chamomile aqueous extract on 4T1 and 47D cells and level of caspase3 protein in breast cancer cells T-47D. World Family Medicine. pp. 62-67. ISSN 1839-0188

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Abstract

Introduction: Breast cancer is one of the most common cancers of women in the world. Many methods have been devised to treat breast cancer, such as radiation therapy, surgery and chemotherapy. Clinical effectiveness of chemotherapy has been limited due to side effects, toxicity and drug resistance. The studies have shown that some medicinal herbs have anti-cancer effects which do not cause side effects of chemotherapy. One of these herbs is Chamomile, the anti-cancer effect of which is being studied by researchers. The effect of Chamomile aqueous extract on cellular survival of breast cancer cells T-47D, 4T1 and protein level of caspase3 in breast cancer cells T-47D has been investigated in this study. Methodology: Breast cancer cells 4T1 and T-47D were treated by blue extract of Chamomile with various concentrations, for 24 hours and then using MTT method, the rate of cytotoxic effect of Chamomile was checked on the above mentioned cells and following that the apoptotic effect of Chamomile extract on treated cells was investigated using ELISA method for determining the amount of caspase3 protein. Results: This study showed that Chamomile extract significantly reduced breast cancer cells survival, concentration-dependent, compared to the control group. Dependent on concentration and time, the Chamomile extract also increased caspase3 protein, indicating induction of apoptosis in the mentioned cells. Conclusion: Chamomile extract, dependent on concentration and time, was able to kill tumour cells that can arise from increasing the level of caspase3 protein. These results can be used in future studies on the anti-cancer effect of Chamomile extract.

Item Type: Article
Keywords: breast cancer Chamomile extract caspase3 cell lines of 4T1 and T-47D blood mononuclear-cells natural-killer activity General & Internal Medicine
Subjects: QW Microbiology and Immunology > QW 501-949 Immunology
Divisions: Education Vice-Chancellor Department > Faculty of Medicine > Department of Basic Science > Immunology Department
Page Range: pp. 62-67
Journal or Publication Title: World Family Medicine
Journal Index: ISI
Volume: 16
Number: 7
Identification Number: https://doi.org/10.5742/mewfm.2018.93473
ISSN: 1839-0188
Depositing User: مهندس مهدی شریفی
URI: http://eprints.dums.ac.ir/id/eprint/127

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